期刊论文

Screening of Shuanghuanglian Injection allergenic ingredients based on immune fingerprint

Liu, H.-Y.

中文

中国中药杂志

1001-5302

2019

44

8

1588-1595

10.19540/j.cnki.cjcmm.20190325.301

国家自然科学基金青年项目(81803729) 湖南省自然科学基金项目(2016JJ2097).

本校为第一且通讯机构

该文采用免疫指纹图谱法,以酶联免疫吸附法（ELISA）结合HPLC/MS法筛查双黄连注射剂（SHLI）的致敏性成分,制备SHLI的鼠抗兔血清免疫球蛋白E抗体（抗Ig E抗体）,通过ELISA包埋抗Ig E抗体可成功吸附SHLI及其不同给药途径的含药血浆样品中的致敏原,提示SHLI可引起大鼠产生Ⅰ型超敏反应。建立抗Ig E抗体吸附前后各样品的HPLC指纹图谱和质谱图,由HPLC指纹图谱的相似度和MS图谱归纳结果可知,不同给药方式可改变SHLI的致敏性,SHLI及其含药血浆中可被特异性抗Ig E抗体吸附的成分有22种,多为酸类、酯类及含氮化合物,基于超分子理论推断这些酸类、酯类及含氮化合物来源于SHLI或机体,可能形成超分子半抗原...

In this paper,immune fingerprint was used to screen the allergenic components of Shuanghuanglian Injection(SHLI) by enzyme-linked immuno sorbent assay(ELISA) combined with HPLC/MS method. ELISA-embedded anti-IgE antibody could successfully adsorb allergens in SHLI and its plasma samples containing drugs through different routes of administration,suggesting that SHLI can induce type I hypersensitivity in rats. HPLC fingerprints and MS map of SHLI and drug-containing plasma samples from different routes of administration before and after anti-IgE antibody adsorption were established. According t...