期刊论文

中文

中国中药杂志

1001-5302

2024

49

04

1073-1081

10.19540/j.cnki.cjcmm.20231114.706

82104809:国家自然科学基金 :深圳市科技计划47号项目 :湖南中医药大学双一流学科建设项目 2022CX29:湖南中医药大学研究生创新课题

本校为第一机构

研究柴胡-白芍含药血浆通过微小RNA-1297(microRNA-1297,miR-1297)/第10号染色体缺失的磷酸酶及张力蛋白同源基因(phosphatase and tensin homologue deleted on chromosome 10,PTEN)信号轴对肝癌细胞HepG2的影响及作用机制.通过实时荧光定量聚合酶链反应(real-time quantitative PCR,RT-qPCR)检测miR-1297、PTEN mRNA在肝癌细胞株中的表达,双荧光素酶报告实验验证miR-1297与PTEN的靶向关系.将HepG2肝癌细胞分为空白血浆组、含药血浆组、miR-1297 inhibitor组、miR-NC组、miR-1297 inhibitor+含药血浆组.细胞计数试剂(CCK-8)法检测细胞增殖,筛选含药血浆最佳干预浓度及时间.细胞划痕实验和Trans...

The present study aimed to investigate the effect and mechanism of Bupleuri Radix-Paeoniae Radix Alba medicated plasma on HepG2 hepatoma cells by regulating the microRNA-1297(miR-1297)/phosphatase and tensin homologue deleted on chromosome 10(PTEN)signaling axis.Real-time quantitative PCR(RT-qPCR)was carried out to determine the mRNA levels of miR-1297 and PTEN in different hepatoma cell lines.The dual luciferase reporter assay was employed to verify the targeted interaction between miR-1297 and PTEN.The cell counting kit-8(CCK-8)was used to de...