期刊论文

中文

中华中医药学刊

1673-7717

2009

27

2

362-364

10.13193/j.archtcm.2009.02.139.hedm.059

湖南省科技计划重点资助项目(2007FJ2005)；

本校为第一机构

目的：通过观察寿胎丸对体外小鼠Th1高分化的CD4^＋T细胞SOCS1和SOCS3蛋白表达的影响,探讨寿胎丸治疗反复自然流产的分子生物学机制。方法：以OVA及IL-12刺激CD4＋T细胞,建立Th1高分化的CD4^＋T细胞模型,按药物浓度和作用时间的不同将细胞模型分为A、B两组,其中A组又分为空白血清对照组和寿胎丸低、中、高剂量药物血清组,B组分为0h、1h、2h、4h和8h组,W estern b lotting方法分别检测SOCS1和SOCS3蛋白的表达。结果：寿胎丸中剂量和高剂量药物血清可显著降低Th1高分化的CD4＋T细胞SOCS1蛋白的表达（P〈0.05）,同时高剂量药物血清还可显著提高其SOCS3蛋白表达水平（P〈0.05）;寿胎丸高剂量药物血清...

Objective：To study molecular biological mechanism of Shoutai Pill treating RSA by observing the effects of Shoutai Pill on the protein expression of SOCS1 and SOCS3 in mice CD＋4T cell which had a differentiation bias toward Th1 cell in vitro.Methods：Th1 differentiation bias of CD＋4T cell model was established by the activiation of ovalbumin and IL-12.These model cells were randomly assigned to A part according to different concentration of drugs and B part according to different reaction times.A part included blank serum contrast group and ...