期刊论文

Construction of an Acute Liver Damage Animal Model for Priming TGF-β/Smad Signal Conduction Pathway in Hepatic Tissue of Rats

中文

中国比较医学杂志

1671-7856

2010

20

7

5-9,后插1-后插2

10.3969/j.issn.1671-7856.2010.07.002

湖南省科技计划项目（NO：2007fj4176） 湖南省卫生厅中医药科研基金（NO：2006103）

本校为第一机构

目的 在传统CCl4急性肝损伤模型的基础上,构建启动大鼠肝组织中的TGF-β/Smad信号传导通路的急性肝损伤动物模塑.方法 将30只大鼠随机分为3组,每组各10只,分别为模型组,对照组和空白组,模型组大鼠予小动脉夹夹闭肝总动脉15 min手术处理,随后分别在第6天和第10天,予25%CCl4花生油溶液6 mL/kg体重腹腔注射;对照组则单用两次CCl4,空白组不做任何处理;第二次CCl4 48 h后处死所有动物.结果 模型组与对照组及空白组比较:血清指标ALT、AST、HA显著上升(P

Objective To construct an acute liver damage animal model for priming TGF-β/Smad signal conduction pathway in hepatic tissue of rats on the basis of traditional model induced by carbon tetrachloride （ CCl4 ）. Methods Thirty rats were randomly divided into three groups as model group,control group and blank group. The model group was prepared by clamping the hepatic artery communis with bulldog clamp for 15 minutes was injected intraperitoneally with 25% of CCl4 contained in arachis oil on the following sixth and tenth day after the operation...