期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

2016

42

4

419-423

10.13331/j.cnki.jhau.2016.04.014

81573951:国家自然科学基金 K1508025-21:长沙市科技局项目

本校为第一机构

为研制新型微生态制剂,从试验小鼠中分离出1株肠道酵母菌,采用形态学、生理生化、基因测序和系统发育树分析等方法对其进行鉴定。结果显示,该菌菌落呈白色、圆形,在显微镜下细胞呈椭圆形,出芽生殖;该菌能发酵葡萄糖、蔗糖和D–甘露醇,能够同化蛋白胨和硝酸钾,不能耐受高浓度乙醇,脲酶试验结果为阴性。序列分析结果表明,该菌株18S rDNA(登录号为KC534843)与汉逊德巴利酵母(Debaryomyces hansenii)18S rDNA(登录号为AB013567)的同源性达99%,26S rDNA(登录号为KC534842)与汉逊德巴利酵母(Debaryomyces hansenii)26S rDNA(登录号为EU131182)的同源性达99%。系统发育树分析结果显示,该菌株18S rDNA与...

In order to develop a new type of probiotics, a yeast strain was isolated from intestine of mice, and it was identified using the methods of morphology, physiology, biochemistry, gene sequencing and phylogenetic tree. The results showed that the bacterial colony presented in white milky color and oval shape. Through microscopic observation, we found its cell presented in oval shape, and the asexual reproduction was gemmation. The yeast could ferment glucose, sucrose and D–mannitol, as well as assimilate peptone and potassium nitrate, while, it...