A fluorescent assay for sensitive detection of kanamycin by split aptamers and DNA-based copper/silver nanoclusters

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成果类型：

期刊论文

作者：

Liu, Yan;Guan, Baibing;Xu, Ziqi;Wu, Yaohui;Wang, Yonghong;...

通讯作者：

Yonghong Wang<&wdkj&>Ge Ning

作者机构：

[Liu, Yan; Guan, Baibing; Wu, Yaohui; Xu, Ziqi; Wang, Yonghong] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.

[Liu, Yan; Guan, Baibing; Wu, Yaohui; Xu, Ziqi; Wang, Yonghong] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.

[Ning, Ge] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.

[Liu, Yan] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Peoples R China.

通讯机构：

[Yonghong Wang] H

[Ge Ning] I

Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, 410004 Changsha, China<&wdkj&>International Education Institute, Hunan University of Chinese Medicine, 410208 Changsha, China

语种：

英文

关键词：

Cu/Ag nanoclusters;Fluorescence detection;Kanamycin;Selectivity;Split aptamer

期刊：

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

ISSN：

1386-1425

年：

2023

卷：

286

页码：

121953

DOI：

10.1016/j.saa.2022.121953

基金类别：

Hunan Provincial Natural Science Foundation of China; Scientific Research Fundof Hunan Provincial Education Department; Training Program for Excellent Young Innovators of Changsha; [2021JJ31138]; [20A520]; [kq2106053]

机构署名：

本校为通讯机构

院系归属：

国际教育学院

摘要：

Kanamycin was a group of essential antibiotics generally served in treating infections of animals which leached into the environment residual in food, causing health concerns. Thus, selective and sensitive monitoring of kanamycin was significant for food safety. In this work, split aptamers were used as templates to prepare fluorescent Cu/Ag NCs for detection of kanamycin. According to the impressive affinity of the aptamer to kanamycin, two different detection modes were designed using kanamycin aptamer as a recognition molecule, in which one ...

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A fluorescent assay for sensitive detection of kanamycin by split aptamers and DNA-based copper/silver nanoclusters

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