期刊论文

Promotion of Astragalus membranaceus polysaccharides on intestinal epithelial cell migration via polyamine mediated Ca~(2+)-RhoA signaling pathway

Liu, P.-A.

中文

中草药

0253-2670

2016

47

23

4224-4230

10.7501/j.issn.0253-2670.2016.23.018

国家自然科学基金资助项目（81373540） 湖南省科技重大专项（2014FJ1007） 湖南省教育厅优秀青年项目（158179） 湖南中医药大学博士科研启动项目（1001-017） 湖南中医药大学中医诊断学国家重点学科开放基金项目（2015ZYZD19）

本校为第一且通讯机构

目的考察黄芪多糖对小肠上皮细胞(IEC-6)迁移及迁移过程细胞内多胺水平、细胞质游离钙离子([Ca~(2+)]_(cyto))的量、细胞骨架蛋白RhoA表达的影响,探讨黄芪修复胃肠黏膜损伤的疗效机制。方法黄芪药材经水提醇沉、Sevage法去蛋白后得到黄芪粗多糖,运用DEAE纤维素柱分离得到黄芪多糖I、II、III、IV,黄芪多糖I以Sephadex LH-20凝胶柱色谱得到黄芪多糖V。Tips划痕法建立IEC-6细胞损伤迁移模型,柱前衍生高效液相色谱法测定多胺的量,流式细胞仪检测[Ca~(2+)]_(cyto)水平,Western blotting法检测RhoA蛋白表达。考察黄芪多糖对正常IEC-6细胞[未加α-二氟甲基鸟氨酸(DFMO)]和多胺耗竭IEC-6细胞(加入DFMO)迁...

Objective: To study the effect of Astragalus membranaceus polysaccharides (AMP) on migration, intracellular polyamines content, cytosolic free Ca2+ concentration ([Ca2+]cyto), and RhoA protein expression of intestinal epithelial (IEC-6) cells, so as to explore the repairing mechanism of Astragalus membranaceus (AM) on gastrointestinal injury. Methods: AM was extracted by water and precipitated by ethanol, AM crude polysaccharides were obtained after removing protein. AMP I, II, III, and IV were obtained by DEAE cellulose column chromatography. ...