期刊论文

Liu, Y.P.

中文

中国应用生理学杂志

1000-6834

2007

23

2

211-215

10.3969/j.issn.1000-6834.2007.02.017

国家自然科学基金资助项目（39800053） 湖南省卫生厅科研项目（B-200305）

本校为其他机构

目的:探讨降钙素基因相关肽(CGRP)对经脂多糖(LPS)诱导大鼠肺泡巨噬细胞分泌基质金属蛋白酶-9(MMP-9)的影响及其机制.方法:对经LPS诱导的大鼠肺泡巨噬细胞给予不同浓度的CGRP干预,并同时设置对照,分别收集上清液,采用明胶酶谱法测定LPS、CGRP或二者联合干预后大鼠肺泡巨噬细胞分泌MMP-9的变化.结果:①正常肺泡巨噬细胞仅分泌少量MMP-9,各浓度CGRP对其分泌无影响,但经LPS诱导后MMP-9的分泌均明显升高(P＜0.01);②不同浓度的CGRP干预呈剂量依赖方式降低LPS诱导的肺泡巨噬细胞MMP-9的分泌(P＜0.01).③CGRP下调LPS诱导的肺泡巨噬细胞MMP-9分泌的作用可为蛋白激酶C阻断剂H-7及钙调蛋白阻断剂W-7部分逆...

AIM: To explore the effects of calcitonin-gene-related peptide (CGRP) on LPS-induced MMP-9 secretion by alveolar macrophages (AM) in vitro. METHODS: The supernatant of LPS-induced Wistar rat AM from different intervention groups were collected to measure the activity by gelatin zymography. RESULTS: (Only secreting a small amount of MMP-9 with unstimulated AM, LPS stimulated MMP-9 production in a concentration-dependent manner (p < 0.01). (2) The activity of MMP-9 in CGRP intervention groups at different levels were significantly lower than those in non-intervention group (p < 0.01). (3) The in...