期刊论文

HPLC determination of the equilibrium solubility and apparent oil/water partition coefficient of cantharidin

Xia, X.-H.

中文

中国新药杂志

1003-3734

2017

26

10

1185-1188

国家自然科学基金（81573621）；

本校为第一且通讯机构

目的:研究斑蝥素的理化性质,为其动力学性质及新剂型设计提供依据。方法:采用高效液相色谱法(HPLC)测定不同溶剂中斑蝥素的平衡溶解度;采用摇瓶法测定正辛醇-水及缓冲液体系中的油水分配系数;以及测定斑蝥素在酸、碱、氧化性和还原性溶液中的稳定性等。结果:斑蝥素在25 ℃下水中的平衡溶解度为40.5 μg·mL~(-1),在乙腈中溶解度最大(36 927.6 μg·mL~(-1)),在缓冲液中溶解度较小,且加入多数表面活性剂后能增加斑蝥素的溶解度,以氢化大豆卵磷脂增溶能力最强;斑蝥素的表观油水分配系数为2.541 2(lgP=0.405 0);斑蝥素在酸、碱、氧化性和还原性溶液中均不稳定。结论:本文建立的测定方法简单可行。斑...

Objective: To determine the physical and chemical properties of cantharidin so as to provide a basis for investigating its dynamic properties and new dosage form design. Methods: The high performance liquid chromatography (HPLC) method was used to detect the equilibrium solubility of cantharidin in various solutions. The apparent partition coefficients of drugs in the octanol-water and octanol-buffer solution systems were measured by shaking-flask method. The stability of cantharidin in acid, alkali, oxidant and reducing solutions was determine...