期刊论文

Enhanced efficacy of paclitaxel-loaded hyaluronic acid functional emulsion in the treatment of ovarian cancer

Luo, L.

中文

中国新药杂志

1003-3734

2018

27

18

2180-2187

国家自然科学基金资助项目（81774362 81303004）

本校为第一机构

目的:制备紫杉醇( paclitaxel,PTX)的透明质酸固体纳米乳剂( PTX-HSNs)用于递送PTX,以改善卵巢癌治疗效果。方法:采用高压均质设备制造PTX-HSNs,对PTX-HSNs进行材料表征,并在体内外评估其性能。结果: PTX-HSNs平均粒径< 100 nm,PTX包载量3 mg·mL~(- 1),包封率( EE)接近100%。通过PTXHSN和不含乙酰透明质酸的PTX固体纳米乳剂( PTX-SNs)对SK-OV-3(分化簇44 [CD_(44)~+ ])细胞和OVCAR-3( CD_(44)~- )细胞的作用比较,表明PTX-HSN与SK-OV-3细胞的亲和能力比PTX-SNs高上百倍。PTX-HSNs的最大耐受剂量( MTD) > 50 mg PTX·kg~(- 1),比Taxol高2.5倍。小鼠肿瘤移植中显示,PTX-HSN治疗比Taxol治疗毒性小,...

Objective: To fabricate paclitaxel-loaded hyaluronan solid nanoemulsions (PTX-HSNs) for the delivery of PTX to improve ovarian cancer treatment via active tumor targeting. Methods: PTX-HSNs were fabricated using high-pressure homogenization and were lyophilized with d-mannitol. The outside of the PTX-HSNs spheres were coated with hyaluronan. Results: The mean size of the PTX-HSNs was maintained to be less than 100 nm, with a relatively narrow size distribution. The PTX loading content was 3 mg•mL-1, and encapsulation efficiency (EE) was close ...