期刊论文

Effects of Shuangdan Mingmu capsule on the expression of Spred-1 and VEGF in the retina of rats with diabetic retinopathy

Qin, Y.-H.

中文

中国新药杂志

1003-3734

2019

28

23

2893-2898

国家自然科学基金面上项目(81874493) 湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心开放基金(2018YGC07) 中医药防治眼耳鼻喉疾病湖南省重点实验室开放基金(2018YZD07)。

本校为第一且通讯机构

目的:观察双丹明目胶囊对糖尿病视网膜病变( diabetic retinopathy,DR)大鼠视网膜组织中出芽相关蛋白-1( sprouty-related protein 1,Spred-1)和血管内皮生长因子( vascular endothelial growth factor, VEGF)表达的影响,探讨其可能的治疗机制。方法: SD大鼠经一次性尾静脉注射STZ( 50 mg·kg~(-1) )诱导糖尿病模型,并随机分为模型组、双丹明目胶囊高、低剂量组、导升明组,同时以正常大鼠为对照。给药结束后, Elisa试剂盒法检测大鼠血浆VEGF含量,HE染色观察大鼠视网膜组织病理改变情况,免疫荧光双标法检测视网膜BrdU/VEGF阳性细胞数,Western Blot法检测大鼠视网膜Spred-1和VEGF蛋白表达情况。结...

Objective: To observe the effects of Shuangdan Mingmu capsule on the expression of sprouty-related protein 1 (Spred-1) and vascular endothelial growth factor (VEGF) in the Retina of rats with diabetic retinopathy (DR) and to explore its possible mechanism. Methods: SD rats were injected with STZ (50 mg•kg-1) via the tail vein to induce diabetes mellitus, and randomly divided into model group, high dose, low dose of Shuangdan Mingmu capsule groups and Doxium group with normal rats as controls. After the last dose, plasma VEGF levels in rats wer...