期刊论文

The effect of Xiaoyao Kangai Jieyu Formula on expression of NMDAR related protein in hippocampus of mice with breast cancer related with depression

Wang, Y.-H.

中文

中国新药杂志

1003-3734

2019

28

5

628-634

湖南省自然科学基金青年项目(2017JJ3235,2018JJ33) 湖南省教育厅优秀青年资助项目(17B201) 湖南省教育厅创新平台开放基金项目(16K067)。

本校为第一且通讯机构

目的:研究逍遥抗癌解郁方对乳腺癌并发抑郁小鼠海马中N-甲基-D-天冬氨酸受体(NMDAR)相关蛋白表达的影响。方法:构建乳腺癌并发抑郁症模型,设立空白组、模型组、紫杉醇组、逍遥抗癌解郁方组、紫杉醇联合氟西汀组和紫杉醇联合逍遥抗癌解郁方组。悬尾实验和强迫游泳实验检测各组小鼠抑郁样行为,免疫组化和实时荧光定量PCR检测小鼠海马NR2A,NR2B及cAMP结合蛋白(cAMP response element binding protein,CREB)与钙调蛋白激酶Ⅱ(calmodulin kinase Ⅱ,CaMKⅡ)蛋白和mRNA的表达以及NR2A, NR2B在肿瘤组织中的相对表达量。结果:与空白组比较,模型组小鼠不动时间显著上升(P < 0.01或P < 0.05),海马中NR2A,N...

Objective: To investigate the effects of Xiaoyao Kangai Jieyu Formula on the expression of N-methyl-D-aspartate receptor (NMDAR) related proteins in hippocampus of breast cancer related with depression mice. Methods: Construction of breast cancer related with depression model, and establishment control group, model group, paclitaxel group, Xiaoyao Kangai Jieyu Formula group, paclitaxel combined with fluoxetine group, and paclitaxel combined with Xiaoyao Kangai Jieyu Formula group. Tail suspension test and forced swimming tests were used to dete...