期刊论文

中文

湖南中医药大学学报

1674-070X

2016

36

4

19-24

10.3969/j.issn.1674-070X.2016.04.005

国家自然科学基金项目资助（81273665） 湖南中医药大学中医诊断学国家重点学科开放基金项目（201401）.

本校为第一机构

目的研究建立大肠癌（colorectal cancer,CRC）)唾液蛋白质指纹图谱新型分子诊断模型。方法采集34例肠癌患者、45例健康志愿者（正常组）的唾液标本,用弱阳离子交换型（WCX）纳米磁珠联合基质辅助激光解析电离飞行时间质谱（matrix assisted laser desorption ionization time-of-flight mass spectrometry,MALDI-TOF MS）技术进行检测,获得各标本的蛋白指纹图谱。用Biomarker Wizard软件分析所获得的蛋白指纹图谱找出差异蛋白,再用Biomarker Patterns 5.0.2建立鉴别诊断模型。结果共检测到312个肠癌差异蛋白质峰,两组比值大于3（肠癌/正常对照>3,或者正常对照/肠癌>3）,其中有37个差异蛋白质峰...

〔Abstract〕 Objective To establish a novel molecular diagnostic model of saliva protein fingerprint in colorectal cancer (CRC) patients. Methods Saliva samples from 34 patients with CRC, and 45 healthy people were analyzed by weak cationic-exchange magnetic beads (MB-WCX) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) methods. Subsequently, we compared the saliva peptide signatures of the two groups and obtained differently expressed peptides by using of Biomarker Wizard, then establish a diagno...