期刊论文

Effect of Poly(amidoamine) on the Phagocytosis of Peritoneal Macrophages in Rats

中文

湖南中医药大学学报

1674-070X

2017

37

7

726-729

10.3969/j.issn.1674-070X.2017.07.008

国家自然基金项目(81373701) 湖南中医药大学青年教师科研基金项目(99820001-199)

本校为第一机构

目的 研究聚酰胺-胺(poly-amidoamine,PAMAM)对大鼠腹腔巨噬细胞吞噬功能的影响。方法 采用CCK-8试剂盒检测PAMAM对巨噬细胞的细胞毒性。通过巨噬细胞吞噬印度墨汁实验和吞噬鸡红细胞实验,观察PAMAM对巨噬细胞吞噬功能的影响。采用扫描电子显微镜(SEM)观察巨噬细胞吞噬鸡红细胞的形貌。结果 0.1-10 mg/m L的G5 PAMAM-NH2和10 mg/m L的G4.5 PAMAM-COOH对巨噬细胞有毒性(P0.05)。0.01 mg/m L的PAMAM对巨噬细胞吞噬鸡红细胞形貌、吞噬率和吞噬指数均无影响(P>0.05)。结论 PAMAM的浓度在0.01 mg/m L范围内,未观察到其对大鼠腹腔巨噬细胞吞噬功能有影响。

Objective To investigate the effect of poly(amidoamine), or PAMAM on the phagocytosis of peritoneal macrophage in rats. Methods The cytotoxicity of the PAMAM to the macrophage was detected by CCK-8 kit. The effect of PAMAM on the phagocytosis of the peritoneal macrophage was observed by the experiments of macrophages swallowing the India ink and chicken erythrocytes. The scanning electron microscope(SEM) was used to observe the morphology of the macrophage phagocytosis of chicken erythrocytes. Results There was toxicity to the macrophages with the concentrations of 0.1-10 mg/m L G5PAMAM-NH2 an...