期刊论文

Experimental study on high-performance liquid chromatography fingerprint of Carthamus tinctorius

中文

湖南中医杂志

1003-7705

2013

29

3

110-112

10.16808/j.cnki.issn1003-7705.2013.03.067

国家科技部“重大新药创制”科技重大专项基金（编号:2011ZX09401-404-14）；

本校为第一机构

目的:利用高效液相色谱建立红花的色谱指纹图谱.方法:运用Agilent 1100 DAD-HPLC高效液相色谱仪,采用Kromasil 100-5 C18(4.6mm×250mm,5μm)色谱柱,乙腈-0.1％磷酸梯度洗脱,流速1.0mL/min,检测波长270nm,柱温25℃,进样量10μL,对10批红花进行试验.结果:10批红花中获得17个色谱共有峰,不同产地红花药材指纹图谱相似度有一定差别.结论:采用HPLC方法建立的红花药材指纹图谱,方法稳定、可靠、简便,色谱图展现的各峰分离度较好,特征明显,可作红花药材真伪鉴别标准,但不同种质红花之间存在一定量及质的差异,说明固定种质来源对保障红花质量的稳定性较为重要.

Objective：To establish the chromatographic fingerprint of Carthamus tinctorius by high-performance liquid chromatography（HPLC）.Methods：HPLC was performed on 10 batches of Carthamus tinctorius using Agilent 1100 DAD-HPLC system.In the HPLC,Kromasil 100-5 C18 column（4.6 mm×250 mm,5μm） was used;the mobile phase was acetonitrile-0.1% phosphoric acid for gradient elution;the flow rate was 1.0 mL/min;the detection wavelength was 270 nm;the column temperature was 25℃;the sample size was 10 μL.Results：Seventeen HPLC common peaks were acquire...