期刊论文

Determination of myricetrin and gallic acid in Sedum aizoon produced in different places

中文

湖南中医杂志

1003-7705

2014

30

4

158-160

10.16808/j.cnki.issn1003-7705.2014.04.084

本校为第一机构

目的:采用高效液相色谱法(HPLC)分析不同产地景天三七中有效成分杨梅苷及没食子酸的含量.方法:以Hypresil BDS C18(4.6mm×250mm,5μm)为色谱柱,流动相分别为乙腈-0.1％磷酸溶液(22∶78)、(1∶99),流速1.0mL/min,柱温30℃;检测波长分别为256nm、273nm.结果:2种成分均在20min内得到较好分离,杨梅苷和没食子酸的进样量分别在0.0880 ～0.5280μg、0.2080 ～1.2480μg范围内与峰面积线性关系良好.平均加样回收率分别为99.4％、99.0％,RSD分别为1.29％、1.94％.结论:本方法简便,准确,重复性好,可作为景天三七的质量控制方法.

Objective：To analyze the content of myricetrin and gallic acid as effective components in Sedum aizoon produced in dif- ferent places by high- performance liquid chromatography （HPLC）. Methods： HPLC was performed on a Hypresil BDSC18（4.6mm×250mm,5μm） with a mobile phase of acetonitrile - 0. 1% phosphoric acid （22：78,1 ： 99） at a flow rate of 1.0 ml/min, a column temperature of 30℃, and detection wavelengths of 256 nm and 273 nm. Results ：Myricetrin and gallic acid were well separated within 20 min. The sample amounts of myricetrin...