期刊论文

中文

湖南中医杂志

1003-7705

2015

31

2

122-124

10.16808/j.cnki.issn1003-7705.2015.02.068

国家自然科学基金资助项目（编号:81273816）；

本校为第一机构

目的:从雌激素/雌激素相关受体信号通路探讨壮骨止痛方抗绝经后骨质疏松症的作用机制.方法:将60只雌性SD大鼠建立去卵巢骨质疏松症病理模型,将其随机分为壮骨止痛方高、中、低剂量组,模型组,雌二醇组,每组12只;另将12只雌性SD大鼠只切除卵巢附近等体积的脂肪组织作为假手术组.各组分别采用相对应药物进行灌胃治疗,治疗13周后各组随机选取6只雌鼠提取股骨组织mRNA样本进行PCR反应检测ERRα基因的表达,选取8只雌鼠经腹主动脉采血,凝固后离心分离出血清,应用雌二醇(E2) ELISA试剂盒检测血清E2水平.结果:与假手术组比较,模型组E2浓度及骨组织ERRα基因表达均显著降低,差异有统计学意义(P＜0.01);与模...

Objective： To investigate the action mechanism of Zhuanggu Zhitong prescription in the treatment of postmenopausal osteoporosis from the perspective of estrogen / estrogen- related receptor signaling pathway. Methods：Sixty female Sprague- Dawley（ SD） rats were ovariectomized to establish a rat model of osteoporosis. And then they were equally and randomly divided into high- dose,middle- dose,and low- dose Zhuanggu Zhitong prescription groups,model group,and estradiol group（ n = 12 for each）. Another 12 female SD rats,from which only the s...