期刊论文

Effect of puerarin on the JAK2/STAT3 signaling pathway in rats after cerebral ischemia/reperfusion injury

中文

湖南中医杂志

1003-7705

2017

33

4

144-147+165

10.16808/j.cnki.issn1003-7705.2017.04.070

湖南省中医药管理局资助项目（编号：201653） 湖南省教育厅研究生创新项目（编号：CX2015B338）

本校为第一机构

目的:探讨葛根素在大鼠脑缺血再灌注损伤后的神经保护作用机制以及与JAK2/STAT3信号通路的相关性.方法:将36只SD雄性大鼠随机分为假手术组12只和造模组24只(其中造模组分缺血再灌注组12只及葛根素组12只).采用线栓法制备大鼠MCAO模型,于脑缺血2h再灌注24h时用Longa评分法进行神经功能缺损评分,TTC染色法测定脑梗死体积,TUNEL法检测海马组织神经细胞凋亡数,免疫组织化学法检测海马组织磷酸化的JAK2(P-JAK2)、STAT3(P-STAT3)的表达.结果:与假手术组比较,造模组神经功能缺损加重,梗死体积增大,凋亡细胞数增多,P-JAK2和P-STAT3表达水平增高,差异具有统计学意义(P＜0.05);与缺血再灌注组比较,葛根素组...

Objective：To investigate the mechanism of neuroprotective effect of puerarin in rats after cerebral is- chemia/reperfusion injury and its association with the JAK2/STAT3 signaling pathway. Methods ： A total of 36 male Sprague - Dawley rats were divided into sham - operation group with 12 rats and model group with 24 rats, and the model group was further divided into ischemia/reperfusion group with 12 rats and puerarin group with 12 rats. The suture method was used to establish a rat model of middle cerebral artery occlusion. At 2 hours of cer...