期刊论文

中文

湖南中医杂志

1003-7705

2017

33

10

157-161

10.16808/j.cnki.issn1003-7705.2017.10.074

湖南省中医药科研计划项目（编号:2015108）；

本校为第一机构

目的:探讨半夏泻心汤对脾虚便秘小鼠肠道细菌多样性的影响.方法:将24只小鼠随机分成4组,每组各6只.分别为正常组、模型组、枳术汤组和半夏泻心汤组,构建小鼠脾虚便秘模型.造模结束后,半夏泻心汤组、枳术汤组分别采用半夏泻心汤和枳术汤灌胃,模型组和正常组均采用蒸馏水灌胃,共6d.治疗结束后提取肠道微生物总DNA,行16S rDNA V4区测序分析多样性.结果:半夏泻心汤和枳术汤对肠道细菌群落的多样性和丰度均无明显影响.各实验组在群落门水平下比较,差异均无统计学意义(P＞0.05).在群落纲水平下Bacilli相对丰度水平上,枳术汤组与正常组比较相对丰度减小(P＜0.05);在Erysipelotrichi相对丰度水平上,枳术...

Objective ： To investigate the effect of Banxia Xiexin decoction on intestinal bacterial diversity in mice with spleen -deficiency constipation. Methods：Twenty -four mice were randomly divided into normal group, model group, Zhizhu decoction group, and Banxia Xiexin decoction group, with 6 mice in each group. A mouse model of spleen- deficiency constipation was established in all mice except those in the normal group. Then, the Banxia Xiexin decoction group and Zhizhu decoction were given Banxia Xiexin decoction and Zhizhu decoction, respecti...