Objective: To investigate the method for establishing a rat model of myocardial fibrosis(MF) using coronary artery ligation,and to provide an experimental basis for clinical prevention and treatment of MF. Methods:Preoperative preparations included experimental animals,drugs,instruments,and devices. The surgery followed the steps of anesthesia,disinfection,connection with a respirator,ECG monitoring,thoracotomy,ligation,closure of the thoracic cavity,ventilator weaning,and postoperative treatment. The surgery required aseptic operation and ...