期刊论文

中文

湖南中医杂志

1003-7705

2018

34

9

155-158

10.16808/j.cnki.issn1003-7705.2018.09.070

国家自然科学基金资助项目（编号:81470192）； 湖南省教育厅科学研究重点项目（编号:13A065）； 湖南省重点学科开发基金项目（编号:4982-0009005）；

本校为第一机构

目的:研究月华丸（胶囊）对小鼠单核巨噬细胞（RAW264.7）活性的影响,为后续研究月华丸（胶囊）对耐多药结核分支杆菌感染细胞自噬的影响提供前期实验基础。方法:取对数生长期的RAW264.7细胞,用10%胎牛血清培养制成的细胞悬液加入96孔培养板中培养。分别采用终浓度为1.25g/m L、0.63g/m L、0.32g/m L、0.16g/m L、0.125g/m L、0.063g/m L、0.032g/m L、0.016g/m L不含阿胶的月华丸（胶囊）以及相同浓度梯度含阿胶的月华丸（胶囊）处理RAW264.7细胞,并设不加药的对照组。以4-甲偶氮唑蓝（MTT）法检测细胞存活情况。结果:（1）不含阿胶的月华丸（胶囊）在1.25g/m L、0.63g/m L浓度时对RAW264.7细胞...

Objective： To investigate the effect of Yuehua pills/capsules on the viability of mouse mononuclear macrophages（ RAW264. 7）,and to provide a preliminary experimental basis for the research on the effect of Yuehua pills/capsules on the autophagy of cells with multidrug-resistant Mycobacterium tuberculosis infection. Methods：RAW264. 7 cells in the logarithmic growth phase were selected,and cell suspension was prepared with 10% fetal bovine serum and then cultured in a 96-well plate. RAW264. 7 cells were treated with Yuehua pills/capsules with...