目的:基于抗氧化活性评价优选禾本科芦笋多糖酶法提取工艺。方法:以活性部位总多糖浸出量与DPPH自由基清除率为评价指标进行评价,在单因素试验的基础上进行正交试验,优选禾本科芦笋多糖的提取工艺。结果:最佳酶法提取工艺为芦笋粉末加3. 0%纤维素酶、p H 6. 5的柠檬酸缓冲溶液,在45℃温浸提取1. 5h,多糖提取量与DPPH自由基清除率分别达到66. 58mg/g与65. 24%。结论:正交试验法优选禾本科芦笋多糖的酶法提取工艺可行,该工艺为禾本科芦笋多糖的酶法提取优化提供了试验依据。
摘要(英文)：
Objective: To optimize the enzymatic extraction process of polysaccharides from asparagus based on antioxidant activities. Methods: With the amount of total polysaccharides from active parts and clearance rate of the free radicals of 2, 2-diphenyl-1-picrylhydrazyl-hydrate (DPPH) as the assessment indices, an orthogonal test was performed based on single-factor experiment to optimize the extraction process of polysaccharides from asparagus. Results: The optimal enzymatic extraction process was asparagus powder with 3. 0％ cellulase and citric ac...
