期刊论文

中文

湖南中医杂志

1003-7705

2019

35

2

130-132

10.16808/j.cnki.issn1003-7705.2019.02.059

国家自然科学基金项目(编号:81470192) 湖南省教育厅科学研究重点项目(编号:13A065) 湖南省重点学科开发基金资助项目(编号:4982-0009005).

本校为第一机构

目的:研究月华胶囊对耐多药结核分枝杆菌感染后细胞自噬的影响,以探寻其可能的抗耐多药结核分枝杆菌作用机制。方法:以月华胶囊含药血清作用于耐多药结核分枝杆菌感染的小鼠单核巨噬细胞（RAW264. 7）,观察其对感染后细胞自噬的影响。实验分组:（1）阴性对照组（耐多药结核分枝杆菌+小鼠单核巨噬细胞）;（2）月华胶囊含药血清组;（3）月华胶囊含药血清+自噬抑制剂3-甲基腺嘌呤（3-MA）组;（4）阳性对照雷帕霉素组;（5）正常空白组。指标检测:在透视电镜下观察月华胶囊含药血清对耐多药结核分枝杆菌感染的小鼠单核巨噬细胞（RAW264. 7）自噬体出现和形成的影响。结果:透射电镜显示,阴性对照组、空...

Objective :To investigate the effec t of Yuehua capsules on cell ciutophagy after niultiding - resistant Mycobaderiuin tuberculosis infection and the possible mechanism of action of Yuehua capsules in the treatment of such inft'dion. Methods :The serum containing Yuehua capsules was used to act on mouse mononuclear macrophages( R AH 264. 7 ) wilh multidrug - resisttml Mycohaclerium tuberculosis infection , in order to observe the effed of Yuehua capsules on cell autophagy after infection. The groups in this experiment were negative control group (multidrug - resistant Mycobacterium tuberculosi...