期刊论文

Effect of Wuhu decoction on the expression of transcription factors in naive CD4^＋T cells in lung tissue in young rats with virus-induced asthma

中文

湖南中医杂志

1003-7705

2018

34

1

148-150

10.16808/j.cnki.issn1003-7705.2018.01.069

湖南省科技计划资助项目（编号：2015JC3076） 湖南省自然科学基金资助项目（编号：13JJ3105）

本校为第一机构

目的：探讨五虎汤对病毒诱发幼年SD哮喘大鼠肺组织初始CD4^＋T细胞转录因子mRNA及蛋白表达的影响。方法：采用RSV诱导,OVA致敏加激发制备大鼠哮喘模型。造模成功后,随机分成五虎汤高、中、低（4.752g/kg、2.376g/kg、1.188g/kg）剂量组,阳性对照组（地塞米松片,2.4mg/kg）,哮喘模型组和空白对照组6组。治疗14d后,取肺组织,分别采用RT-PCR及免疫印迹法检测肺组织初始CD4^＋T细胞转录因子T-bet、GATA-3、RORγt、Foxp3 mRNA及其蛋白表达情况。结果：模型组T-bet、Foxp3 mRNA及蛋白较正常对照组表达降低（P〈0.05）,GATA-3、RORγt mRNA及蛋白较正常对照组表达升高（P〈0.05）;与模型组比较,五虎汤...

Objective： To investigate the effect of Wuhu decoction on the mRNA and protein expression of transcription factors in naive CD4^＋T cells in lung tissue in young Sprague-Dawley rats with virus-induced asthma. Methods： A rat model of asthma was established by RSV induction and OVA sensitization and challenge. After the model was successfully established,the rats were randomly divided into low-,medium-,and high-dose Wuhu decoction groups（ 4. 752,2. 376,and 1. 188 g/kg,respectively）,positive control group（ dexamethasone tablets,2. 4 mg/kg）,a...