期刊论文

The Effect of Spleen-strengthening Therapy on t-PA and PAl and Col IV in Rats with Cerebral Ischemia/Reperfusion Injury

中文

中国中医急症

1004-745X

2016

25

12

2213-2215,2267

10.3969/j.issn.1004-745X.2016.12.002

81202632:国家自然科学基金 81473567:国家自然科学基金 20124323120003:高等学校博士学科点专项科研基金 13JJ3097:湖南省自然科学基金 14B134:湖南省教育厅优秀青年项目 15K092:湖南省教育厅高校创新平台基金项目

本校为第一机构

目的 观察健脾补土法组方对脑缺血/再灌注损伤大鼠t-PA、PAI-1、ColⅣ的影响,探讨其保护神经细胞可能作用机制.方法 将72只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组、健脾补土方小剂量组、健脾补土方中剂量组、健脾补土方大剂量组,每组12只.采用线栓法制备MCAO模型,治疗7d后处死,并在大鼠处死前进行神经功能缺损评分,应用酶联免疫吸附(ELISA)法测定中组织型纤溶酶原激活物(t-PA)及组织型纤溶酶原激活物抑制物-1(PAI-1)的变化,运用免疫组化法测定胶原蛋白Ⅳ(ColⅣ)的含量变化.结果 与模型组相比,健脾补土小、中、大剂量组神经功能缺损症状评分减少(P＜0.05);健脾补土方小、中、大剂量组t...

Objective： To study the effect of Spleen-strengthening therapy on t-PA,PAI-1 and Col IV and in rats with cerebral ischemia/reperfusion injury and investigate its possible mechanism. Methods： 72 male SD rats were randomly divided into sham-operation group,modelling group,edaravone group and low-dosed spleen- strengthening group,middle-dosed spleen-strengthening group,high-dosed spleen-strengthening group, 12 in each group： The models of MCAO were induced by middle cerebra/artery occlusion and recanalization executed after treatment of 7 days,...