期刊论文

中文

中国药房

1001-0408

2013

24

35

3286-3288

10.6039/j.issn.1001-0408.2013.35.07

本校为第一机构

目的:研究薯蓣皂苷元对人肝L-02细胞胆固醇代谢的影响,并初步探讨胆固醇转运蛋白(Caveolin-1)在其中的表达变化.方法:正常人肝L-02细胞与50％胎牛血清共同孵育24h,以复制脂变肝细胞模型.不同质量浓度薯蓣皂苷元(0、20、40、80 μmol/L)作用于细胞24h与40μmo/L薯蓣皂苷元作用于细胞不同时间(0、6、12、24、48 h),油红O染色观察细胞内脂滴情况,高效液相色谱法检测细胞内总胆固醇(TC)、游离胆固醇(FC)、胆固醇酯(CE)的含量,Western blot法检测Caveilin-1蛋白的表达.结果:0、20、40、80 μmol/L浓度下,薯蓣皂苷元可减少脂变人肝L-02细胞内TC、FC、CE含量,同时Caveolin-1蛋白表达上调,并呈现一定的剂...

OBJECTIVE：To study the effects of diosgenin on cholesterol metabolism in human hepatocyte L-02,and to investigate the expression of caveolin-1.METHODS：Human hepatocyte L-02 was incubated with 50% fetal bovine serum for 24 h to induce fatty degeneration model of hepatic cells.After exposed to different concentrations of diosgenin（0,20,40,80 μmol/L）for 24 h and diosgenin 40 μmo/L for 0,6,12,24 and 48 h,lipid the lipid droplet of hepatocyte L-02 was observed by oil red O staining.The contents of TC,FC and CE were determined by HPLC,and the p...