期刊论文

Content Determination of Ecdysterone in Different Parts of Radix serratulae by HPLC

中文

中国药房

1001-0408

2016

27

15

2147-2149

10.6039/j.issn.1001-0408.2016.15.44

本校为第一机构

目的：建立测定广升麻植株中不同部位蜕皮甾酮含量的方法。方法：采用高效液相色谱法。色谱柱为ZORBAX Eclipse XDB-C18，流动相为甲醇-水（46∶54，V/V），流速为1.0 ml/min，检测波长为248 nm，柱温为30℃，进样量为10μl。比较广升麻植株不同部位中蜕皮甾酮的含量。结果：蜕皮甾酮的检测进样量线性范围为0.12～1.21μg；精密度、稳定性、重复性试验的RSD＜1.5％；加样回收率为98.4％～101.9％，RSD＝1.64（n＝6）。广升麻植株根中的蜕皮甾酮含量最高，其次是块茎，茎和叶的含量较低；种子中则不含蜕皮甾酮成分。结论：该方法简便快速、准确性和重复性好，适用于测定广升麻植株中不同部位蜕皮...

OBJECTIVE：To establish a method for the content determination of ecdysterone in different parts of Radix serratulae. METHODS：HPLC was performed on the column of ZORBAX Eclipse XDB-C18 with mobile phase of methanol-water at a flow rate of 1.0 ml/min（46 ∶ 54,V/V）,detection wavelength was 248 nm,column temperature was 30℃,and the injection volume was 10 μ l. Compare the contents of ecdysterone in different parts of R. serratulae. RESULTS：The linear range of ecdysterone was0.12-1.21 μg;RSDs of precision,stability and reproducibility tests ...