期刊论文

中文

中国药房

1001-0408

2018

29

4

461-465

10.6039/j.issn.1001-0408.2018.04.08

湖南省科技计划滚动项目（No.2015sk1001-1） 湖南省高校“2011协同创新中心”项目（No.湘财教指〔2015〕69号）

本校为第一机构

目的:建立枳壳药材挥发油的气相色谱-质谱(GC-MS)指纹图谱.方法:采用GC-MS法,色谱柱为RTX-5MS毛细管柱,进样口温度为250℃, 载气为高纯氦气(≥99. 999％),流速为1. 0 mL/min,分流比为10:1,进样量为1 μL(程序升温);采用电子轰击离子源,离子源温度为230 ℃,检测器温度为250 ℃,溶剂延迟时间为3 min,扫描范围为m/z35~550.以月桂烯为参照,测定21批药材挥发油样品的GC-MS图谱,采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行相似度评价,确定共有峰,采用LC Solution2质谱库(NIST05. LIB和NIST05s. LIB)确定共有峰成分,并采用面积归一化法测定共有峰的相对含量.结果:21批药材挥发油样品的GC-MS...

OBJECTIVE: To establish GC-MS fingerprint of volatile oil from Citrus aurantium. METHODS: GC-MS method was adopted. The determination was performed on RTX-5MS capillary column with injector temperature of 250 ℃, high pure helium as carrier gas(≥99. 999％), flow rate of 1. 0 mL/min, split ratio of 10:1,and sample size of 1 μL (temperature programming). Mass spectrum condition included electron bombardment ion source, ion source temperature of 230 ℃, detector temperature of 250 ℃, 3 min solvent delay, scanning range of m/z 35-550. GC-MS chro...