期刊论文

Effect of dexmedetomidine on oxidative stress responses during global cerebral ischemia-reperfusion in rats

中文

中华麻醉学杂志

Chinese Journal of Anesthesilolgy

1673-923X

2015

35

3

377-379

10.3760/cma.j.issn.0254-1416.2015.03.030

本校为第一机构

目的评价右美托咪定对大鼠全脑缺血再灌注时氧化应激反应的影响。方法健康雄性SD大鼠36只,体重250~300 g,采用随机数字表法分为3组(n= 12):假手术组(S组)、全脑缺血再灌注组(I/R组)和右美托咪定组(D组)。采用夹闭双侧颈总动脉联合低血压法制备大鼠全脑缺血再灌注损伤模型。D组于再灌注即刻经颈总静脉注射右美托咪定3 μg/kg负荷剂量,随后以3 μg?kg~(-1) ?h~(-1)的速率静脉输注至再灌注2 h。于再灌注24 h时行神经功能缺陷评分(NDS评分),然后处死大鼠,取脑组织,计数凋亡细胞,计算细胞凋亡率,测定丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的水平。结果与S组比较,I/R组和D组NDS评分、细胞...

Objective To evaluate the effects of dexmedetomidine on the oxidative stress responses during global cerebral ischemia-reperfusion （I/R） in rats. Methods Thirty-six male Sprague-Dawley rats, weighing 250-300 g, were randomly divided into 3 groups （n= 12 each） using a random number table： sham operation group （group S）, global cerebral I/R group （group I/R） and dexmedetomidine group （group D）. Global cerebral ischemia was induced by occlusion of bilateral common carotid arteries com- bined with hypotension （MAP maintained at 35-45 mm...