期刊论文

Zheng, J.-H.

中文

中国组织工程研究

2095-4344

2013

17

36

6515-6520

10.3969/j.issn.2095-4344.2013.36.021

国家自然科学基金项目（81102595）《养心通脉有效部位方对BMSCs作用靶点及信号转导通路的研究》 广西自然科学基金项目（2012GXNS即A053113）《心血瘀阻证心肌微环境变化及对骨髓间充质干细胞移植的影响》

本校为第一且通讯机构

背景：黄芪甲苷和丹参酮Ⅱa 是传统中医药治疗心肌缺血的有效成分，是否可参与骨髓间充质干细胞向心肌样细胞分化的过程呢？　　目的：观察黄芪甲苷联合丹参酮Ⅱa对体外诱导骨髓间充质干细胞向心肌样细胞分化作用的影响。　　方法：应用MTT法分别测定黄芪甲苷和丹参酮Ⅱa的最大无毒浓度，确定两者联合诱导骨髓间充质干细胞分化为心肌样细胞的剂量。将分离纯化的骨髓间充质干细胞分5组进行诱导：黄芪甲苷组、丹参酮Ⅱa 组、丹参酮Ⅱa+黄芪甲苷组、5-氮胞苷组、空白对照组，ELISA方法观察体外诱导后骨髓间充质干细胞缝隙连接蛋白43和肌钙蛋白表达变化。　　结果与结论：诱导后丹参酮Ⅱa+黄芪甲苷组...

BACKGROUND:Astragaloside and tanshinone IIa are the effective components of traditional Chinese medicine treatment of myocardial ischemia, and its role in bone marrow mesenchymal stem cel s differentiation into myocardium-like cel s remains unclear. OBJECTIVE:To investigate the effect of tanshinone IIa and astragaloside on the differentiation of bone marrow mesenchymal stem cel s into myocardium-like cel s. METHODS:The maximal non-toxic concentrations of tanshinone IIa and astragaloside were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, to define the dose o...