Background: Caveolin-1 is expressed in mammalian brain and involved in the normal development of the brain, which can affect the proliferation of neural stem cells in the brain. Objective: To acquire neural stem cells from caveolin-1 knockout embryonic mice in vitro and study their biological characteristics. Methods: The whole brain was separated from C57BL/6 mice and caveolin-1 knockout C57BL/6 mice respectively at encyesis 14-16 days. Single cell suspension was obtained by enzyme digestion, and cultured in the conditioned medium of neural stem cells. Following 7 days of primary culture, the...