期刊论文

中文

中国组织工程研究

2095-4344

2014

18

23

3739-3744

10.3969/j.issn.2095-4344.2014.23.022

国家自然科学基金(30873355,81273989) 国家重点基础研究发展计划(973计划)(2010CB530400) 2012年度湖南省高校创新平台开放基金项目(12K089)~~

本校为第一机构

背景:研究发现细胞质膜微囊蛋白1在哺乳动物脑内表达,参与脑的正常发育,能影响脑内神经干细胞的增殖。目的:从细胞质膜微囊蛋白1敲除小鼠脑内获取神经干细胞并观察其生物学特性。方法:分别取E14-E16正常C57BL/6胚胎小鼠和细胞质膜微囊蛋白1敲除C57BL/6胚胎小鼠全脑,采用酶消化法获得单细胞悬液,置神经干细胞条件培养基中培养,原代培养7 d后,加入含体积分数10%胎牛血清的DMEM/F12培养基诱导7 d。结果与结论:从两种胎鼠脑内获取的细胞悬液培养1 d后较多细胞发生死亡,可见单个细胞漂浮于培基中,透光度较好,3 d后逐渐形成悬浮生长的多细胞团。传代后培养板底部可见少量细胞发生贴壁,7 d后可见大量细...

Background: Caveolin-1 is expressed in mammalian brain and involved in the normal development of the brain, which can affect the proliferation of neural stem cells in the brain. Objective: To acquire neural stem cells from caveolin-1 knockout embryonic mice in vitro and study their biological characteristics. Methods: The whole brain was separated from C57BL/6 mice and caveolin-1 knockout C57BL/6 mice respectively at encyesis 14-16 days. Single cell suspension was obtained by enzyme digestion, and cultured in the conditioned medium of neural stem cells. Following 7 days of primary culture, the...