期刊论文

An Experimental Study on the Effects of DLXNP on the Gerbils Models of Cerebral Ischemia and Reperfusion

中文

中国中医药信息杂志

1005-5304

2003

10

5

29-31

10.3969/j.issn.1005-5304.2003.05.015

本校为第一机构

目的:研究丹龙醒脑片(DLXNP)对脑缺血再灌注沙土鼠模型脑组织兴奋性氨基酸(EAAs)含量的影响及对海马区神经元的保护作用.方法:用双侧颈总动脉反复夹闭再灌注方法建立脑缺血再灌注沙土鼠模型.用高效液相色谱分析荧光法(HPLC)检测脑组织谷氨酸(Glu)、天门冬氨酸(Asp)浓度,用病理光学显微镜观测其双侧海马CA1区神经元数目.结果:模型组与正常对照组和假手术组相比,G1u、Asp含量明显升高(P＜0.01),海马区神经细胞数明显减少(P＜0.01).丹龙醒脑片3个剂量组脑组织中G1u、Asp的含量明显低于模型组(P＜0.01～0.05),海马区神经细胞数明显多于模型组(P＜0.01).结论:丹龙醒脑片能调节缺血再灌注大鼠脑组织兴...

Objective: To investigate the intervenient effects of DLXNP on cerebral EAAs and its protection effects on hippocampal cells following cerebral ischemia and reperfusion in the gerbils models. Methods : The ischemia and reperfusion model was made by repeatedly occludding bilateral carotid arteries (CCA) with artery clips for two times, every time for 5 minutes, brains were recirculated for 10 minutes by removal of clips between the two times and after the last occludding. Samples were extracted from hippocampal region and then made paraffin section and HE staining so as to make observation on h...