期刊论文

Determination of eight active constituents in Kendir leaves tablets Ⅰ by HPLC-QAMS by single marker

中文

药物分析杂志

0254-1793

2019

39

8

1433-1442

10.16155/j.0254-1793.2019.08.13

湖南中医药大学一流学科项目(201803) 国家自然科学基金面上项目(81573621)

本校为第一机构

目的:建立同时测定复方罗布麻片Ⅰ中8个活性成分含量的一测多评法(QAMS)。方法:采用HPLC法,以氢氯噻嗪为内参物,分别计算其与金丝桃苷、粉防己碱、防己诺林碱、绿原酸、蒙花苷、盐酸异丙嗪、硫酸双肼屈嗪的相对校正因子,通过相对校正因子计算复方罗布麻片Ⅰ中7个活性成分的含量(计算值),同时采用外标法测定8个活性成分的含量(实测值),比较计算值与实测值的差异。色谱柱为Kromasil C_(18) (250 mm×4.6 mm,5 μm),流动相为乙腈-0.5%磷酸溶液(梯度洗脱),流速0.7 mL·min~(-1),检测波长260 nm(氢氯噻嗪、盐酸异丙嗪、硫酸双肼屈嗪、金丝桃苷)、280 nm(粉防己碱、防己诺林碱)、325 nm(绿原酸、蒙花苷...

Objective:To establish the quantitative analysis of multi-components by single marker(QAMS)for simultaneous determination of 8 active constituents in Kendir leaves tabletsⅠ. Methods:Using hydrochlorothiazide as internal standard,HPLC method was used to calculate relative correction factor(RCF)of hyperoside, tetrandrine,fangchinoline,chlorogenicacid,buddleodide,promethazine,dihydralazine sulfate. The contents of above-mentioned 7 active components in Kendir leaves tablets Ⅰ were calculated by using RCF(calculation value). The external standard...