期刊论文

中文

北京中医药大学学报

1006-2157

2014

37

12

812-815

10.3969/j.issn.1006-2157.2014.12.005

No.81102593:国家自然科学基金 81273753:国家自然科学基金 No.20110491258:中国博士后科学基金

本校为第一机构

目的探讨滋阴益气活血解毒中药含药血清对脂肪酸孵育HepG2细胞中固醇调节元件结合蛋白 - 1c(SREBP-1c)和脂肪酸合成酶(FAS)表达的影响。方法HepG2细胞分为对照组、模型组、15% 含药血清组;对照组和模型组用正常大鼠血清 DMEM 培养,15% 含药血清组用15% 含药血清 DMEM 培养,模型组、15% 含药血清组同时加0.5 mmol / L 混合脂肪酸(油酸 ∶ 棕榈酸 = 2∶ 1);油红 O 染色法检测细胞内脂质沉积,RT-PCR 检测HepG2细胞 SREBP-1c 和 FAS mRNA 表达,免疫组化检测HepG2细胞 SREBP-1c 和 FAS 蛋白表达。结果15% 含药血清能减少模型细胞脂质沉积,降低模型细胞 SREBP-1c 和 FAS 的 mRNA 及蛋白表达,差异具有统...

Objective To investigate the effects of serum with yin-enriching,qi-replenishing,and bloodactivating Chinese medicine on the expressions of SREBP-1 c and FAS in steatotic HepG2 cells.Methods HepG2 cells were randomly divided into the control group,model group,and 15％ medicated serum group.The control group and model group were cultured with normal rat serum DMEM while the 15％ medicated serum group was given 15％ medicated serum DMEM.Moreover,mixed fatty acids （oleic acid,hexadecanoic acid =2：1） （0.5 mmol/L） were added to the model group ...