期刊论文

Regulatory effect of Zhuanggu Zhitong Capsule on Treg /Th17 balance in rats with osteoporosis induced by ovariectomy

中文

北京中医药大学学报

1006-2157

2016

39

7

555-561

10.3969/j.issn.1006-2157.2016.07.007

国家自然科学基金面上项目（No.81273816）；

本校为第一机构

目的从骨组织调节性T细胞/辅助性T细胞(Treg /Th17)平衡角度探讨壮骨止痛胶囊抗绝经后骨质疏松作用机理。方法72只8月龄SD雌鼠随机分为假手术组,模型组,戊酸雌二醇组(0.21 mg /kg), 壮骨止痛胶囊高剂量组(1.28 g /kg) 、中剂量组(0.64 g /kg)和低剂量组(0.32 g / kg), 每组12只。除假手术组外,其余5组去卵巢复制绝经后骨质疏松动物模型。术后1周开始给药,连续给药18周,最后一次给药1 h后处死,快速取左股骨检测雌二醇(E_2) 、白细胞介素17A(IL-17A) 、白细胞介素10(IL-10), 取右股骨RT-PCR检测维甲酸相关孤体受体γt(ROR_(γt)) 、叉状头转录因子(Foxp3) 、IL-17A、IL-10 mRNA的表达,取左胫骨病理...

Objective To investigate the anti-postmenopausal osteoporosis mechanism of Zhuanggu Zhi- tong Capsule （Bone-strengthening Pain-relieving Capsule, ZGZTC ） in regulating Treg/Thl7 balance in bone tissue. Methods Seventy-two 8-month-old SD female rats were allocated randomly into sham oper- ation group, model group, estradiol group （0.21 mg/kg）, ZGZTC high-dose group （1.28 g/kg）, ZGZ- TC medium-dose group （0.64 g/kg） and ZGZTC low-dose group （0.32 g/kg）. There were 12 rats in each group. Bilateral ovariectomy was performed on rats in all...