期刊论文

中文

北京中医药大学学报

1006-2157

2018

41

1

60-64,75

10.3969/j.issn.1006-2157.2018.01.010

湖南省教育厅科研项目 National Natural Science Foundation of China 国家自然科学基金项目 Education Department of Hunan Province

本校为第一机构

目的以缺氧诱导的HK-2细胞为研究对象,探讨脯氨酸羟化酶2 /缺氧诱导因子1α(PHD2 / HIF-1α)信号途径对肾间质纤维化的影响,以及六味地黄汤含药血清干预作用。方法40只SPF级雄性SD大鼠分为空白组和给药组,每组20只,空白组以10 mL /(kg·d)生理盐水,给药组以67.5 g /(kg·d)六味地黄汤灌胃连续1周,腹主动脉取血,离心后分离血清。体外培养HK-2细胞,将实验分为空白对照组(10%胎牛血清) 、正常血清组(10%正常大鼠血清) 、氯化钴(CoCl_2)处理组(150 μmol /LCoCl_2 + 10%正常大鼠血清)和含药血清组(150 μmol /LCoCl_2 + 10 %六味地黄汤含药血清) 。体外培养HK-2,分组处理24 h后,用Western Blot法和R...

Objective To investigate the effect of PHD2/HIF-1 signaling pathway on the renal interstitial fibrosis, and the intervention effect of medicated serum of Liuwei Dihuang Tang on HK-2 cells induced by hypoxia. Methods A total of 40 SPF male SD rats were divided into blank group and medicated group （each n = 20）. The blank group was orally given normal saline [ 10 mL/（ kg·d）] and medicated group was orally given Liuwei Dihuang Tang for 1 week continuously, and then blood samples were collected from rat abdominal aorta for separating serum aft...