期刊论文

Effects of Mimenghua Granule on IL - 12 and IL - 6 in lacrimal gland cells of castrated male rabbits with induced dry eyes

中文

北京中医药大学学报

1006-2157

2019

42

6

477-482

10.3969/j.issn.1006-2157.2019.06.008

No.30772824:国家自然科学基金 No.81574031:国家自然科学基金 No.2017TP1018:湖南省重点实验室建设项目 No.K1501014-31:长沙市科技计划 No.kc1704005:长沙市科技计划 :中央财政支持地方高校建设项目 :国家中医药管理局中医药重点学科建设 :湖南省中医五官科学重点学科建设项目

本校为第一机构

目的 观察密蒙花颗粒对去势雄兔泪腺细胞白细胞介素6(IL-6)及白细胞介素12(IL-12)表达的影响.方法 30只新西兰长耳白兔随机平均分为5组:正常组、模型组、密蒙花颗粒组、生理盐水组、睾酮组.通过切除双侧睾丸及附睾造模.给药30 d后,进行基础泪液分泌试验(SIT)和泪膜破裂时间(BUT)检测,然后将所有兔处死,摘取泪腺组织行HE染色,观察泪腺组织结构,免疫组化法检测各组兔泪腺组织中IL-6、IL-12的表达.结果 正常组兔泪腺组织排列整齐,结构清晰,未见IL-6、IL-12表达;模型组及生理盐水组泪腺组织排列不整齐,结构大片变性,IL-6、IL-12大量表达于细胞膜和细胞浆中;密蒙花颗粒组及睾酮组泪腺组织排列整齐,结...

Objective To observe the effects of Mimenghua (Flos Buddlejae) Granule (MG) on the expressions of IL-6 and IL-12 in lacrimal gland cells of castrated male rabbits. Methods Thirty New Zealand long-eared white rabbits were randomly and evenly divided into 5 groups: normal group,model group,MG group,saline group and testosterone group. Except rabbits in the normal group,all the others were castrated by bilateral orchiectomy and epididymectomy. SIT and BUT test were performed 1 day before and 30 days after castration. After 30 days of intervention,all rabbits were executed. Lacrimal gland tissues ...