期刊论文

Influence and mechanism of Danshen Tongluo Jiedu Tang combined with bone marrow stem cell transplantation on myocardial fibrosis in rats with acute myocardial infarction

中文

北京中医药大学学报

1006-2157

2019

42

8

655-661

10.3969/j.issn.1006-2157.2019.08.008

国家自然科学基金资助项目(No.81704065) 湖南省自然科学基金资助项目(No.2019JJ40210) 湖南省教育厅科学研究重点资助项目(No.15A143) 湖南省中医药科研计划资助项目(No.201559,No.201790) 长沙市科技计划资助项目(No.kq1706058).

本校为第一机构

目的探讨丹参通络解毒汤联合骨髓干细胞移植对急性心肌梗死(AMI)大鼠心肌纤维化的影响及作用机制。方法采用全骨髓贴壁法结合密度梯度离心法分离和培养骨髓间充质干细胞(BMSCs) 。经结扎冠状动脉的左前降支(LAD)建立心梗模型。大鼠随机分为假手术组、AMI模型组、BMSCs移植组、中药组(丹参通络解毒汤组) 、联合组(丹参通络解毒汤+ BMSCs移植组) 。除假手术组外其余组造模,术后中药组与联合组分别灌胃丹参通络解毒汤,其余各组用等量生理盐水灌胃,1次/d,连续14 d。Masson染色观察心肌组织胶原含量,ELISA法测定血清Ⅲ型前胶原末端肽(PⅢP) 、Ⅳ型前胶原末端肽(PⅣP) 、一氧化氮(NO) 、内皮素-1(ET-1...

Objective To investigate the influence and mechanism of Danshen Tongluo Jiedu Tang (Salvia Root Collateral-freeing Removing Toxicity Decoction) combined with bone marrow stem cell transplantation (BMSCT) on myocardial fibrosis in rats with acute myocardial infarction (AMI). Methods The bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured by using whole bone marrow adherence method combined with density gradient centrifugation. The model of AMI was established through ligating the left anterior descending branch (LAD) of coronar...