期刊论文

Effects of Jingui Shenqi Decoction on hypoxia due to renal fibrosis in rats and its mechanism

中文

北京中医药大学学报

1006-2157

2019

42

9

753-759

10.3969/j.issn.1006-2157.2019.09.009

国家自然科学基金青年项目(No.81603470) 湖南中医药大学一级学科基础医学建设项目。

本校为第一机构

目的探讨金匮肾气汤对单侧输尿管梗阻( UUO)大鼠肾间质纤维化缺氧的影响及作用机制。方法雄性SD大鼠24只,随机分为假手术组、模型组、金匮肾气汤组、依那普利组,每组6只,采用左侧输尿管结扎术造模,UUO术后第1天开始灌胃,金匮肾气汤1.8 g /kg,依那普利10 mg / kg,每日1次。第21天处死大鼠,取梗阻肾组织,HE和Masson染色观察肾组织病理改变,免疫荧光检测Tomato Lectin、Hypoxyprobe-1和缺氧诱导因子-1α( HIF-1α)在肾组织的表达,Western Blot检测转化生长因子-β1( TGF-β1) 、骨形态发生蛋白( BMP-7)和HIF-1α在肾组织的表达。结果与模型组比较,金匮肾气汤治疗后,HE和Masson染色结果提示梗阻肾肾...

Objective To investigate the effect of Jingui Shenqi ( Golden Cabinet's Kidney Qi) Decoction on hypoxia in unilateral ureteral obstruction ( UUO) rats and its mechanism. Methods 24 male SD rats were randomly divided into 4 groups: sham operated group,UUO model group,Jingui Shenqi Decoction ( JSD) treatment group,and enalapril ( ENP) treatment group with 6 rats in each group. The models were established by left ureter ligation. From the first day after UUO,the JSD group and ENP group were given intragastrically JSD ( 1.8 g /kg) and enalapril ( 1...