期刊论文

Protective effect and its mechanism of Naotaifang on rat cortical neurons injury induced by hemoglobin

中文

北京中医药大学学报

1006-2157

2019

42

10

825-832

10.3969/j.issn.1006-2157.2019.10.006

No.81774174:国家自然科学基金 No.81774033:国家自然科学基金 No.201969:湖南省中医药科研项目 No.2018ZXYJH15:湖南中医药大学中西医结合一流学科开放基金项目

本校为第一机构

目的 探讨脑泰方对体外血红蛋白(Hb)诱导的大鼠皮质神经元损伤的保护作用及其机制.方法 原代培养新生SD大鼠皮质神经元,采用Hb诱导神经元模拟建立脑出血后神经元损伤细胞模型;实验设正常组、模型组、空白血清对照组、去铁胺组和10％脑泰方含药血清组.采用CCK8法检测各组细胞活力;采用Calcein-AM染色法检测各组细胞内铁含量;采用免疫荧光与高内涵细胞成像分析技术(HCA)检测各组神经元转铁蛋白(Tf)及其受体(TfR)、二价金属离子转运体(DMT-1)蛋白表达情况;采用酶联免疫吸附法检测各组上清液中脂质活性氧自由基(lipid-ROS)含量;采用real-time PCR法检测各组Tf、TfR和DMT-1 mRNA表达水平.结果 与正常...

Objective To investigate the protective effect and its mechanism of Naotaifang ( Brain Blood-activating Formula, NTF)on rat cortical neurons injury induced by hemoglobin (Hb) in vitro. Methods Primarycortical neuronsfrom newborn SD rats were cultured and induced by Hb( 10micmol/L) to simulate iron overload of neurons after ICH. The cultured neurons were randomly divided into the normal group, model group, blank serum group, 10% NTF gmedicated serum roup and deferoxamine ( DFX) group. Neuron viability was detected by CCK8 assay. The content of iron in neurons was detected by Calcein-AM staining...