期刊论文

Intervention of Compound Uncaria Tablets on myocardial differential protein in spontaneously hypertensive rats based on iTRAQ technology

中文

北京中医药大学学报

1006-2157

2020

43

02

133-140

10.3969/j.issn.1006-2157.2020.02.007

No.81774207:国家自然科学基金 No.2019ZX09301103:重大新药创制"科技重大专项 No.2018JJ2291:湖南省自然科学基金面上项目

本校为第一机构

目的研究复方钩藤降压片干预自发性高血压大鼠(SHR)心肌组织蛋白质变化及高血压左心室肥厚潜在机制。方法将16只SHR分为2组后,治疗组予以复方钩藤降压片灌胃干预,对照组予以蒸馏水灌胃,干预12周后采取大鼠心肌组织蛋白质进行同位素标记相对和绝对定量(iTRAQ)检测分析。通过对富集Pathway分析,得到差异蛋白及通路,并将得到的差异蛋白利用STRING数据库绘制差异蛋白功能联系。结果差异蛋白富集Pathway分析提示存在11条通路。差异蛋白中上调蛋白6种,分别为:转胶蛋白、碳酸酐酶3、S期激酶相关蛋白1、C反应蛋白、纤维蛋白5、C-1-四氢叶酸合成酶;下调蛋白12种,分别为:谷胱甘肽S-转移酶、Ighg3蛋白、组氨...

Objective To study the protein changes in the intervention of the Compound Uncaria Tablets in Spontaneously Hypertensive Rats (SHR) and the potential mechanism of left ventricular hypertrophy in hypertension. Methods 16SHRs were divided into two groups. The treatment group was gavaged with the Compound Uncaria Tablets,and the control group was gavaged with distilled water. After 12 weeks of intervention,the myocardial tissue protein was taken and tested by iTRAQ assay analysis. By analyzing the enrichment of pathways,the differential proteins and pathways were obtained,and furtherly mapped usi...