期刊论文

Regulating effect and mechanism of Zuogui Jiangtang Jieyu Formulation on linkage damage of hippocampal neurovascular unit in rats with diabetes- related depression

中文

北京中医药大学学报

1006-2157

2020

43

11

918-926

10.3969/j.issn.1006-2157.2020.11.006

No.2017ZX09309026:重大新药创制"科技重大专项 No.81573965:国家自然科学基金 No.81874464:国家自然科学基金 No.20200165:湖南省卫生计生委科研项目 No.2018ZYX47:湖南中医药大学中医学一流学科开放基金 No.2018-2020:湖南中医药大学中医学国内一流建设学科项目

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目的 探讨左归降糖解郁方对糖尿病并发抑郁症(DD)大鼠海马神经血管单元(NVU)联动损伤的调控作用及其潜在机制.方法 原代分离、纯化和培养SD大鼠海马神经元(Ne)、星形胶质细胞(As)与脑微血管内皮细胞(Bm),并对其进行免疫荧光染色鉴定;采用高糖联合皮质酮及Transwell小室干预构建DD海马NVU细胞模型;实验设正常组、模型组、空白血清组、左归降糖解郁方含药血清组.造模及给药18 h后,检测海马NVU跨内皮细胞电阻值(TEER)及4 h渗漏试验液面高度值;采用ELISA法分别检测Bm分泌蛋白白血病抑制因子(LIF)及As分泌蛋白转化生长因子 β(TGF-β)、胶质细胞源性神经营养因子(GDNF)含量;采用免疫荧光联合高内涵细...

Objective To investigate the regulating effect and mechanism of Zuogui Jiangtang Jieyu Formulation (ZGF) on linkage damage of hippocampal neurovascular unit (NVU) in diabetes-related depression. Methods Hippocampal neurons,astrocytes and brain microvascular endothelial cells from SD rats were primitively isolated,purified and cultured,and immunofluorescence staining was used to identify cells. The DD model of hippocampal NVU was established with high dose of glucose and cortisone after hippocampal NVU co-culture system was built by Transwell in...