期刊论文

Mechanism of Compound Qishao Jiangya tablets in the treatment of spontaneously hypertensive rats based on AKT/mTOR signal pathway

中文

北京中医药大学学报

1006-2157

2020

43

04

296-303

10.3969/j.issn.1006-2157.2020.04.006

No.81473616:国家自然科学基金 No.2018SK51203:湖南省科技厅项目 No.201910:湖南省中医药局重点项目 :中医内科重大疾病防治 No.ZYNK201703:教育部重点实验室开放基金 No.2018CX37:创新基金

本校为第一机构

目的探讨复方七芍降压片通过调控蛋白激酶B(AKT)/雷帕霉素靶蛋白(mTOR)信号通路对自发性高血压(SHR)大鼠血压、炎症因子及胸主动脉自噬相关蛋白表达的影响。方法SHR大鼠30只,随机分为模型组、厄贝沙坦片组(27 mg/kg)、复方七芍降压片组(1.73 g/kg),连续给药6周,另设10只WKY大鼠为正常组,灌胃生理盐水。分别在给药前及给药1、2、3、4、5、6周,测量各组大鼠尾动脉血压。给药6周后取材,电镜观察胸主动脉超微结构及自噬情况;ELISA法检测血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)水平;免疫组化法检测胸主动脉中Beclin-1、Bcl-2、 AKT、mTOR蛋白表达;Western-blot检测胸主动脉中Beclin-1、Bc...

Objective To investigate the effects of compound Qishao Jiangya tablet on blood pressure,inflammation factors and expression of aortic autophagy related proteins in spontaneously hypertensive(SHR)rats by regulating protein kinase B(AKT)/rapamicin target protein(mTOR)signaling pathway.Methods Thirty male SHR rats were randomly divided into model group,irbesartan group(27 mg/ kg),and compound Qishao Jiangya tablet group(1.73 g/kg).Another 10 male WKY rats were used as normal control group(saline).All intervention was administered for 6 continuous...