期刊论文

Effect of medicated serum of Naotaifang Ⅱ on microglia polarization induced by LPS

中文

北京中医药大学学报

1006-2157

2020

43

5

408-413

10.3969/j.issn.1006-2157.2020.05.010

国家自然科学青年基金项目(No.81603608、8174174) 教育部博士后面上项目(No.2018M630905) 湖南省自然科学青年基金项目(No.2019JJ50431) 中医内科重大疾病防治研究与转化教育部重点实验室开放基金(No.ZYNK201708) 湖南省大学生研究性学习和创新性实验计划项目(No.1021-0001017161) 湖南中医药大学中医学一流学科开放基金(No.4901-020000200221)

本校为第一机构

目的探明脑泰方Ⅱ号含药血清对LPS诱导的HAPI小胶质细胞形态、M1/M2型极化标记分子及炎性因子的影响。方法建立LPS诱导的HAPI细胞炎症模型,将培养的细胞分为:空白组、LPS模型组、脑泰方Ⅱ号组、米诺环素组。高内涵观察细胞形态与数量的变化,Real-time PCR检测M1型标志分子MHCⅡ、iNOS、MCP-1、CD11b,M2型标志分子Arg1、Mrc1、Ym1,促炎性因子IL-1β、IL-6、IL-12、TNF-α,抗炎性因子IL-4、IL-10、TGF-β mRNA水平。结果与正常组比较,LPS模型组细胞胞体变大,并呈多极化或阿米巴型,突触变粗变短;其M1型标记分子与促炎性因子的mRNA水平显著升高(P

Objective To investigate the influence of medicated serum of NaotaifangⅡon the morphology,M1/M2 polarization markers and inflammatory factors of HAPI cells induced by LPS.Methods The inflammation model of HAPI cell was induced by LPS.The cultured cells were divided into: blank group,model group,Naotaifang Ⅱ group,and minocycline group.Morphology and quantity of microglia were observed by High Content Imaging System.mRNA expression of M1-type markers(MCH II,iNOS,MCP-1,CD11b)and M2-type markers(Arg1,Mrc1,Ym1),pro-inflammatory factors(IL-1β,IL-...