期刊论文

Effects of Liuwei Dihuang Decoction on the expression of HIF-1α,Twist and E-cadherin in renal tissue of 5 /6 nephrectomized rats

中文

北京中医药大学学报

1006-2157

2020

43

11

927-934

10.3969/j.issn.1006-2157.2020.11.007

No.81503442:国家自然科学基金 No.81603470:国家自然科学基金 No.17B197:湖南省教育厅科学研究项目

本校为第一机构

目的观察六味地黄汤对5 /6肾切除大鼠肾脏缺氧诱导因子-1α(HIF-1α) 、Twist和上皮钙黏蛋白E(E-cadherin)表达的影响,探讨六味地黄汤延缓肾纤维化的分子机制。方法60只SD雄性大鼠随机分为空白对照组(10只) 、假手术组(10只)和手术组(40只) 。手术组按5 /6肾切除法造模处理,造模成功后将手术组(死亡1只)随机分为模型组、六味地黄汤组、依那普利组,每组13只。六味地黄汤组予以六味地黄汤6.75 g /kg灌胃,依那普利组予以依那普利10 mg /kg灌胃,空白对照组、假手术组和模型组予以等量的蒸馏水灌胃,每日1次,干预8周。于第8周末采集当日24 h尿液、腹主动脉全血、肾组织,检测各组大鼠肾功能指标,HE染色...

Objective To observe the effect of Liuwei Dihuang Decoction on the expression of HIF-1α, Twist and E-cadherin in 5 /6 nephrectomy rats,and explore the molecular mechanism of Liuwei Dihuang Decoction in delaying renal fibrosis. Methods 60 male SD rats were randomly divided into the control group (n = 10),sham operation group (n = 10) and operation group (n = 40). The model of 5 /6 nephrectomy rats was established in the operation group. After successful modeling,the operation group (one dead) was randomly divided into model group,Liuwei Dihuang...