期刊论文

Effects of Zuogui Jiangtang Shuxin formula medicated plasma on pyroptosis and apoptosis induced by ox-LDL in J774A.1,cells

中文

北京中医药大学学报

1006-2157

2021

44

04

332-339

10.3969/j.issn.1006-2157.2021.04.008

No.81573956:国家自然科学基金 82074400:国家自然科学基金 No.2018YFC1704300:国家重点研发计划 No.2020SK2101:湖南省重点研发计划项目 No.18A220:湖南省教育厅科学研究项目 20K094:湖南省教育厅科学研究项目 No.2018ZYX44:湖南省国内一流建设学科中医学开放基金项目

本校为第一机构

目的研究左归降糖舒心方含药血浆对氧化低密度脂蛋白(ox-LDL)诱导巨噬细胞焦亡及凋亡状态的影响,探讨该方治疗糖尿病动脉粥样硬化斑块的新靶点。方法SD大鼠随机分成3组,连续5 d灌胃相应的药物制备含药血浆:空白组(灭菌超纯水)、左归降糖舒心方组(25.92 g/kg)、西药联合用药组(二甲双胍0.83 g/kg +阿托伐他汀钙12.45 mg/kg)。应用ox-LDL诱导细胞焦亡及凋亡状态。通过CCK-8法比较各组J774A.1细胞增殖情况,计算细胞存活率,筛选含药血浆及ox-LDL干预浓度。将分化完全的J774A.1巨噬细胞随机分为空白血浆组、模型组、左归降糖舒心方含药血浆组、西药联合含药血浆组,每组6个复孔。诱导及给药24 h后,LDH...

Objective To investigate the effects of Zuogui Jiangtang Shuxin (kidney-supplementing glucose-lowering heart-soothing)formula medicated plasma on ox-LDL-induced pyroptosis and apoptosis and to explore new targets in the treatmert of diabetic atherosclerosis with this formula.Methods SD rats were administered intragastrically with 7.2 g/kg Zuogui Jiangtang Shuxin formula or 0.83 g/kg metformin combined with 12.45 mg/kg atorvastatin calcium to prepare drug-containing plasma.Zuogui Jiangtang Shuxin formula medicated plasma was then extracted for J774A.1 cells treatment.J774A.1 cells were pretreat...