期刊论文

Using network pharmacology to study the molecular mechanism and validate the key pathway of Kidney-qi pill against Type 2 diabetes mellitus

中文

北京中医药大学学报

1006-2157

2021

44

01

60-68

10.3969/j.issn.1006-2157.2021.01.010

国家自然科学基金面上项目（No.81573956）； 国家重点研发计划中医药现代化研究重点专项（No.2018YFC1704300）； 湖南省研究生科研创新项目（No.CX2018B471）； 湖南省“国内一流建设学科”中医学开放基金项目（No.2018ZYX44）~~；

本校为第一机构

目的基于网络药理学方法探讨肾气丸治疗2型糖尿病的活性成分、作用靶点及可能作用机制,并通过体外实验进行初步验证。方法应用TCM database@Taiwan、Chemistry Database及TCMSP数据库获取肾气丸的化学活性成分及相关靶点信息,通过对比GenCards及OMIM 2个数据库,归纳预测肾气丸化学成分中明确对2型糖尿病具有治疗作用的靶点,进行成分-靶点相互作用网络图的构建。运用DAVID 6.8数据库对基因富集分析,获得GO功能注释结果及KEGG通路富集结果,分析通路相关基因及功能。综合药物-疾病靶基因,使用String 10.5工具构建靶点相互作用网络,通过Cytoscape软件进行拓扑分析,对肾气丸关键化学成分及靶点进行评...

Objective To explore the active components,core targets and mechanism of Kidney-qi pill in treating for Type 2 diabetes mellitus ( T2DM),based on network pharmacology and preliminarily verify it through in vitro experiment. Methods The TCM database@ Taiwan,Chemistry Database and TCMSP database were used to filter the chemical composition and target of Kidney-qi pill. The corresponding gene name of each target was obtained in UniProt database. Then,the target genes of Kidney-qi pill and T2DM were plotted,depending on the relationship between chemical composition and target. The String software ...