会议论文

中文

2012

281-287

全国第4届中西医结合传染病学术会议

全国第4届中西医结合传染病学术会议论文集

2012-9-1

上海

中国中西医结合学会

本校为第一机构

目的:比较健脾、补肾与解毒法对慢性HBV感染免疫清除期患者外周血DCs介导的T淋巴细胞的影响。方法:分别收集10例HBV慢性感染免疫清除期患者和8例健康人的抗凝外周血,分离外周血单个核细胞（PBMCs）,体外培养DCs,用大鼠健脾、补肾、解毒药物血浆进行干预,并建立DCs、T细胞相互作用的模型,检测T细胞的表面分子CD3+、CD4+、CD8+、CD8+CD28+、CD8+PD-1受体的表达,并检测T细胞上清液中IFN-γ的分泌水平。结果:经补肾、健脾、解毒药物血浆处理,HBcAg负载的DCs孵育的T淋巴细胞,CD3+、CD4+、CD8+CD28+表达率升高,CD8+和CD8+PD-1表达率降低,分泌IFN-γ的能力提高。结论:补肾、健脾、解毒法在一定程度上都...

　　Objective:To compare the effect ofTCM decoctions(Sijunzi Tang,Liuweidihuang Tang and Ganluxiaodu Tang)on T lymphocytes of people in immune clearance stage with chronic HBV infection, which were mediated by peripheral blood DCs.Methods:Ten patients in immune clearance stage with chronic HBV infection and eight healthy controls were included in this study.DCs were differentiated from PBMCs which were isolated from their peripheral blood ,incubated and induced then,the DCs were interfered by plasma of medicine of Liuweidihuang Tang ,Sijunzi Ta...