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Effects of different drying methods on the chemical constituents of Lilium lancifolium Thunb. based on UHPLC-MS analysis and antidepressant activity of the main chemical component regaloside A

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成果类型:
期刊论文
作者:
Yuan, Zhi-ying;Li, Zi-yi;Zhao, Hong-qing;Gao, Chuan;Xiao, Meng-wu;...
通讯作者:
Xu, Guang-Ming;Xie, MZ
作者机构:
[Xiao, Meng-wu; Xie, Meng-zhou; Li, Zi-yi; Xu, Guang-Ming; Yuan, Zhi-ying; Xu, GM; Zhao, Hong-qing; Zhu, Jian-ping; Jiang, Xing-ming; Huang, Hui-yong] Hunan Univ Chinese Med, 301 Xueshi St, Changsha 410208, Hunan, Peoples R China.
[Gao, Chuan] Hunan Agr Prod Proc Inst, Changsha, Peoples R China.
[Xu, Guang-Ming; Yuan, Zhi-ying] Hunan Engn Technol Ctr Standardizat & Funct Chine, Changsha, Peoples R China.
[Xie, Meng-zhou; Yuan, Zhi-ying; Zhu, Jian-ping; Huang, Hui-yong] Key Lab TCM Heart & Lung Syndrome Differentiat &, Changsha, Peoples R China.
通讯机构:
[Xie, MZ ; Xu, GM] H
Hunan Univ Chinese Med, 301 Xueshi St, Changsha 410208, Hunan, Peoples R China.
语种:
英文
关键词:
Amino acids;Cell signaling;Drying;Enzymes;Mammals;Brain-derived neurotrophic factors;Chemical component;Chemical compositions;Chemical constituents;Phosphatidylinositol;Phosphatidylinositol 3-kinase;Target of rapamycin;Tyrosine-kinase receptors;Chemical analysis;herbaceous agent;regaloside A;unclassified drug;antidepressant agent;corticosterone;plant extract;antidepressant activity;Article;BDNF TrkB signaling;cell counting;chemoluminescence;controlled study;drug conformation;electrospray;genetic fingerprinting;human;human cell;ion chromatography;Lilium lancifolium;lily;mass spectrometry;molecular docking;neuroprotection;optical density;PI3K Akt mTOR signaling;SH-SY5Y cell line;signal transduction;survival rate;ultra performance liquid chromatography;Western blotting;cell survival;chemical structure;chemistry;desiccation;drug effect;high performance liquid chromatography;isolation and purification;lily;mass spectrometry;tumor cell culture;Antidepressive Agents;Cell Survival;Chromatography, High Pressure Liquid;Corticosterone;Desiccation;Humans;Lilium;Mass Spectrometry;Molecular Docking Simulation;Molecular Structure;Plant Extracts;Tumor Cells, Cultured
期刊:
JOURNAL OF SEPARATION SCIENCE
ISSN:
1615-9306
年:
2021
卷:
44
期:
5
页码:
992-1004
基金类别:
The authors gratefully acknowledge the financial support of the project of the Changsha Municipal Bureau of Science and Technology (kq1801044), the Youth project of Natural Science Foundation of Hunan Province (2019JJ50446), and the Research project of traditional Chinese medicine in Hunan Province(202108, 202069).
机构署名:
本校为第一且通讯机构
摘要:
The Lilium lancifolium Thunb. is a herb with multiple functions in both medicine and food in China, and its extracts have shown antidepressant effects. In this study, fresh bulbs of Lilium lancifolium Thunb. were processed to study the effects of different drying processes on changes in its main chemical components. We found that different drying methods can affect the chemical constituents of the herb. Among these components, Regaloside A has been found as the characteristic component. Here, Cell Counting Kit-8 assay, and Western blotting were...

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