To obtain a method of determination of the content of solancesol in different parts of tobacco and different materials of tobacco, HPLC method was used to determin the content of solanesol in different parts of tobacco by methanol supersound extract and tobacco extract by different methods. The result showed that the content of solanesol in the stem and leafstalk of tobacco was the lowest,the above leaf of tobacco stem is better,the middle leaf of tobacco stem is the best and the below leaf of tobacco stem is good. The better tobacco is,the higher content of solanesol is. The petroleum ether i...